Rex1 Antibody (14H16L9), ABfinity™ Rabbit Monoclonal

货号：701159

规格：100 µg

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

抗原：Rex1

物种：人

宿主：大鼠

抗体亚型：IgG

克隆号：14H16L9

荧光染料：其它

类型:	单抗	同型对照：	IgG
免疫原性：	Recombinant protein corresponding to amino acids 1–140 of human REX1 transcription factor	用法：	1-3 µg/mL(ICC);1-3µg/mL(IF);2-5 µg/mL(WB)

Product Specific InformationIntact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.Target InformationRex1 (zinc finger protein 42 homolog (ZFP42)) is a member of a class of DNA binding proteins which act as transcriptional regulators of other genes. Specifically, Rex1 is involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. It is required for efficient elongation of TSIX – a non-coding RNA antisense to XIST. Rex1 binds the DXPas34 enhancer within the TSIX promoter. It is also involved in ES cell self-renewal. Rex1 is expressed in healthy kidney, epidermal keratinocytes, prostate epithelial cells, bronchial/small airway lung epithelial cells, and embryonic stem cells. It is also expressed in malignant kidney and several carcinoma cells lines.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Rex1 Antibody (701159) in IFImmunofluorescent analysis of Rex1 in HeLa cells using a Rex1 recombinant rabbit monoclonal antibody (Product # 701159) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Rex1. Rex1 Antibody (701159) in WBWestern blot analysis of Rex1 in A431 whole cell extracts using a Rex1 recombinant rabbit monoclonal antibody (Product # 701159) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~35kDa.

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