ERK1/ERK2 (Phospho) [pT202/pY204] ELISA Kit/ERK1/ERK2 (Phospho) [pT202/pY204]ELISA试剂盒

货号：EMS2ERKP

规格：96 tests

价格：11148

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.67 pg/mL
检测范围	62.5-2000 pg/mL
样本类型/体积	细胞裂解液 12.5 μL
操作时间	1 hr 20 min
测试时间	3 hr
Homogenous (no wash)	No
批间变异系数（CV）	<6.3%
批内变异系数（CV）	<3.7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 检测抗体 -抗兔-HRP - 细胞裂解液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	ERK1/2
Protein aliases	ERK-1, ERK-2, ERT1, ERT2, Extracellular signal-regulated kinase 1, Extracellular signal-regulated kinase 2, extracellular signal-related kinase 1, extracellular-signal-regulated kinase 2, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 2, MAP kinase 3, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Microtubule-associated protein 2 kinase, mitogen activated protein kinase 1, mitogen-activated 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 3, MNK1, p42-MAPK, p44 MAP kinase, p44-ERK1, p44-MAPK, pp42/MAP kinase, protein tyrosine kinase ERK2
物种（Tested）	小鼠，大鼠，人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	9030612K14Rik, AA407128, AU018647, C78273, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, Esrk1, HS44KDAP, HUMKER1A, Mapk, MAPK1, MAPK2, MAPK3, MNK1, Mtap2k, p38, p40, p41, p41mapk, p42-MAPK, P42MAPK, p44, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM1, PRKM2, PRKM3
Gene ID	(Human) 5594,(Human) 5595,(Mouse) 26413,(Rat) 116590,(Rat) 50689,(Mouse) 26417
Gene symbol	MAPK1, MAPK3, Mapk1, Mapk1, Mapk3, Mapk3
UniProt ID	(Human) P28482,(Human) P27361,(Mouse) P63085,(Rat) P63086,(Rat) P21708,(Mouse) Q63844

The ERK1/2 [pThr202/Tyr204] ELISA quantitates IL-1b in cell lysates. The assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204.ERK1 / 2 [pThr202 / Tyr204] ELISA定量细胞裂解物中的IL-1b。 当在苏氨酸202和酪氨酸204上进行磷酸化时，该检测试剂盒将只识别天然和重组ERK 1/2。Principle of the method 方法原理The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.磷酸化ERK1 / 2固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.ERK1和ERK2广泛表达，并参与分化细胞的减数分裂，有丝分裂和有丝分裂后功能的调控。 许多不同的刺激（包括生长因子，细胞因子，病毒感染，异三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）偶联的受体和转化剂的配体）激活ERK1和ERK2途径。 当生长因子与受体酪氨酸激酶结合时，Ras与丝氨酸/苏氨酸蛋白激酶Raf相互作用并对其进行活化。 一旦被激活，Raf使另外2种激酶MEK1 / 2的丝氨酸残基磷酸化，进而使细胞外信号调节激酶（ERK）1/2中的酪氨酸/苏氨酸磷酸化。 激活后，ERK会磷酸化许多细胞质靶标或迁移到细胞核，然后在细胞核中磷酸化并激活许多转录因子，例如c-Fos和Elk-1。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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