Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red-X/山羊抗小鼠IgG (H+L)交叉吸附二抗，Texas Red-X

货号：T-862

规格：1 mg

价格：2629

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Texas Red

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10 µg/mL(ICC);1-10 µg/mL(IF)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (T-862) in IF Actin labeled with BODIPY FL phallacidin (B607) and vinculin, a cytoskeletal focal adhesion protein, tagged with a monoclonal anti-vinculin antibody that was subsequently probed with Texas Red goat anti-mouse IgG antibody (T862). The large triangular cell is a fibroblast containing green actin stress fibers terminating in red focal adhesions. The neighboring polygonal cell, a rat neonatal cardiomyocyte, contains green striated actin in the myofibrils terminating in the focal adhesions. The close apposition of the two stains results in a yellowish-orange color. Image contributed by Mark B. Snuggs and W. Barry VanWinkle, University of Texas, Houston. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (T-862) in IF Photomicrograph of mouse fibroblasts that have been formaldehyde-fixed, acetone-permeabilized and triple-stained with the F-actin-specific probe BODIPY® FL phallacidin (Product # B607), with mouse monoclonal anti-ß-tubulin antibody in conjunction with Texas Red® goat anti-mouse IgG antibody (Product # T-862) and with DAPI (Product # D1306, D3571, D21490). The image was obtained by taking multiple exposures through bandpass optical filter sets appropriate for fluorescein, Texas Red® dye and DAPI using a 100X Plan Apochromat objective.

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参考文献：

1.PloS oneCold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures.2.Breast cancer research : BCRThe small molecule AUTEN-99 (autophagy enhancer-99) prevents the progression of neurodegenerative symptoms.3.Stem cells translational medicineDelivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells.4.PLoS pathogensIntracellular Theileria annulata promote invasive cell motility through kinase regulation of the host actin cytoskeleton.5.Eukaryotic cellA contiguous compartment functions as endoplasmic reticulum and endosome/lysosome in Giardia lamblia.

技术参数

产品应用 ICC;IF;IHC(F);IHC;Misc

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