F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, NANOGOLD/F(ab')2-山羊抗小鼠IgG (H+L)二抗NANOGOLD
货号:N-24915
规格:1 mL
价格:0
产品类型:二抗
品牌:Thermo Fisher
抗原:Gamma Immunoglobin
物种:小鼠
宿主:山羊
抗体亚型:IgG
荧光染料:其它
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抗体类型: | 荧光二抗 | 同型对照: | |
浓度: | 0.08 mg/mL | 用法: | Assay Dependent (ICC);0.2-10 µg/mL(IM) |
产品详细信The NANOGOLD Fab' fragment of goat anti-mouse IgG is covalently conjugated with uniform, 1.4 nm-diameter gold clusters. When used with our LI Silver Enhancement Kit (L-24919), the NANOGOLD conjugates can be used as a probe in immunoblotting, light microscopy or electron microscopy.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
数据 |
Mouse IgG (H+L) Secondary Antibody (N-24915) in IF Spindle microtubules labeled with an anti-tubulin primary antibody followed by (left) goat anti-mouse IgG antibody colloidal gold or (right) NANOGOLD Fab' fragment of goat anti-mouse IgG antibody (Product # N-24915). Micrographs (magnification = 1300x) were generated by D. Vandre and R. Burry, Ohio State University. Image courtesy of Nanoprobes, Inc. |
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参考文献: |
1.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biologyImmunogold labeling of chromosomes for scanning electron microscopy: a closer look at phosphorylated histone H3 in mitotic metaphase chromosomes of Hordeum vulgare.2.Journal of immunological methodsColorimetric detection of protein microarrays based on nanogold probe coupled with silver enhancement.3.Biophysical journalSelective cell targeting with light-absorbing microparticles and nanoparticles.4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyAn antigen retrieval method using an alkaline solution allows immunoelectron microscopic identification of secretory granules in conventional epoxy-embedded tissue sections.5.The Journal of cell biologyVCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo. |
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