Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin-XX/山羊抗小鼠IgG (H+L)交叉吸附二抗，生物素-XX

货号：B-2763

规格：1 mg

价格：2033

产品类型：二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1 µg（ChIP);1-10 µg/mL（ICC);1-10 µg/mL(IF);1:500-1:5,000(WB)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (B-2763) in IF Mouse fibroblast microtubules labeled with a mouse monoclonal anti-ß-tubulin antibody in conjunction with biotin-XX goat anti-mouse IgG antibody (Product # B-2763) and then developed for visualization with alkaline phosphatase-mediated techniques using the ELF® 97 Cytological Labeling Kit (Product # E6603). This kit's novel ELF® 97 phosphatase substrate yields a yellow-green-fluorescent precipitate at the site of alkaline phosphatase activity. Prior to antibody labeling, mouse fibroblasts were fixed and permeabilized in the presence of cytoskeletal stabilizing buffer and treated with paclitaxel (Product # P3456) to stabilize microtubule structures. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (B-2763) in IF Reversible binding by DSB-X™ biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti-a-tubulin antibody (Product # A11126), detected with either biotin-XX goat anti-mouse IgG antibody (Product # B-2763, panel A) or DSB-X™ biotin goat anti-mouse IgG antibody (Product # D-20691, panel B) and visualized with green-fluorescent Alexa Fluor® 488 streptavidin (Product # S-11223). Nuclei were stained with blue-fluorescent DAPI (Product # D1306, D3571, D21490). After incubating with 10 mM d-biotin (Product # B-1595, B20656), the binding between the biotinylated antibody is unaltered (panel C), whereas the streptavidin conjugate has been stripped from the DSB-X™ biotin-labeled antibody (panel D). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (B-2763) in IFThe cortical region of the developing follicle of the giant silkmoth Antheraea polyphemus stained with a monoclonal antibody against cytoskeletal actin. The primary antibody was visualized using biotin-XX goat anti-mouse IgG antibody (Product # B-2763), followed by incubation with Texas Red® streptavidin (Product # S-872). The orange-to-pink colors in this confocal laser-scanning micrograph show the distribution of cytoskeletal actin in the oocyte cortex and follicle cell cytoplasm. The blue color can be attributed to autofluorescence. The image was contributed by Ivo Sauman, Wesleyan University.

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参考文献：

1.Journal of neuroscience methodsA facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts.2.Molecular visionPKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.3.BloodNSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal Vγ2Vδ2 T-cell expansion.4.Journal of proteome researchComparison of hydroxylated print additives on antibody microarray performance.5.Arteriosclerosis, thrombosis, and vascular biologyCell cycle-dependent regulation of smooth muscle cell activation.

技术参数

产品应用 ChIP;ICC;IF;WB;Misc

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