Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC/山羊抗小鼠IgG (H+L)高交叉吸附二抗，FITC

货号：A16079

规格：1 mg

价格：1504

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：FITC

抗体类型:	荧光二抗	同型对照：
浓度：		用法：	1:500-1:20,000 (Flow);1:500-1:20,000（ICC);1:500-1:20,000(IF)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16079) in IFHeLa cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-ATP synthase subunit IF1 antibody (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS. Cells were then incubated with a 1:1000 dilution of FITC-labeled secondary antibody (Product # A16079) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (Product # R37605) and ActinRed™ 555 ReadyProbes® reagent (Product # R37112) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (Product # P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (Product # AMEP4699). Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16079) in IFHeLa cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-ATP synthase subunit IF1 antibody (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS. Cells were then incubated with a 1:1000 dilution of FITC-labeled secondary antibody (Product # A16079) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (Product # R37605) and ActinRed™ 555 ReadyProbes® reagent (Product # R37112) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (Product # P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (Product # AMEP4699). Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16079) in IF HeLa cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-ATP synthase subunit IF1 antibody (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS. Cells were then incubated with a 1:1000 dilution of FITC-labeled secondary antibody (Product # A16079) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (Product # R37605) and ActinRed™ 555 ReadyProbes® reagent (Product # R37112) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (Product # P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (Product # AMEP4699).

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