Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3/山羊抗小鼠IgG（H + L）交叉吸附二抗，Cyanine3

货号：M30010

规格：1 mL

价格：1635

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：其它

类型:	二抗	同型对照：
浓度：		用法：	1 to 400(ICC)；1 to 400(IF)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (M30010) in IFImmunofluorescent analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin (DM1A) Mouse Monoclonal Antibody (Product # 62204) at 1 µg/mL (Panel a), 2.5 µg/mL (Panel b) and 5 µg/mL (Panel c) in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate (Product # M30010) a dilution of 1:400 for 45 minutes at room temperature. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panels a, b and c represent the merged images showing cytoplasmic localization. Panel d is the no primary antibody control. Panel e shows cells labeled with Arfaptin 2 Rabbit Polyclonal Antibody (Product # 40-2400) and Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate (Product # M30010) in order to demonstrate the specificity. The images were captured at 40X magnification Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (M30010) in IFImmunofluorescent analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin (DM1A) Mouse Monoclonal Antibody (Product # 62204) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate (Product # M30010) a dilution of 1:400 for 45 minutes at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c represents the merged image showing cytoplasmic localization. Panel d is the no primary antibody control. Panel e shows cells labeled with Arfaptin 2 Rabbit Polyclonal Antibody (Product # 40-2400) and Goat anti-Mouse IgG (H+L) Secondary Antibody, Cy3 conjugate (Product # M30010) in order to demonstrate the specificity. The images were captured at 60X magnification.

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