Rat anti-Mouse IgG1 Secondary Antibody, FITC, eBioscience™/大鼠抗小鼠IgG1二抗，FITC, eBioscience™

货号：11-4015-82,11-4015-80

规格：100μg,25μg

价格：1474,615

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：FITC

抗体类型:	荧光二抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow);Assay-Dependent (ICC);Assay-Dependent（IF);Assay-Dependent(IHC(F));Assay-Dependent(IHC(P))

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cell after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (11-4015-82) in Flow Staining of normal human peripheral blood cells with staining buffer (blue histogram) or Anti-Human CD4 Purified (RPA-T4, a mouse IgG1 antibody) (Product # 14-0049-82) (purple histogram) followed by 0.25 µg of Anti-Mouse IgG1 FITC. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Scientific reportsPolychlorinated biphenyls target Notch/Dll and VEGF R2 in the mouse placenta and human trophoblast cell lines for their anti-angiogenic effects.2. Cellular immunologyA proliferation-inducing ligand sustains the proliferation of human naïve (CD27⁻) B cells and mediates their differentiation into long-lived plasma cells in vitro via transmembrane activator and calcium modulator and cyclophilin ligand interactor and B-cell mature antigen.3. GastroenterologyExpression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.4. PloS oneProtein kinase D2 is an essential regulator of murine myoblast differentiation.5. Respiratory researchDifferential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages.

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