Goat anti-Mouse IgG (H+L) Poly-HRP Secondary Antibody, HRP/山羊抗小鼠IgG (H+L) Poly-HRP二抗，HRP

货号：32230

规格：500 µL

价格：2448

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified Mouse IgG, whole molecule

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:2500-1:10,000 (ELISA);1:50-1:500（IHC);1:5,000-1:20,000(WB)

产品详细信息This antibody may cross-react with immunoglobulins from other species.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Poly-HRP Secondary Antibody (32230) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Poly-HRP Secondary Antibody, HRP conjugate (Product # 32230) at dilutions 1:2, 500 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. Molecular neurobiologyDexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain.2. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyThe mTORC2/Akt/NFκB Pathway-Mediated Activation of TRPC6 Participates in Adriamycin-Induced Podocyte Apoptosis.3. OncotargetTranscriptome-wide identification of mRNAs and lincRNAs associated with trastuzumab-resistance in HER2-positive breast cancer.4. PloS oneCathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophils.5. Molecular nutrition and food researchCross-talk between microRNAs, nuclear factor E2-related factor 2, and heme oxygenase-1 in ochratoxin A-induced toxic effects in renal proximal tubular epithelial cells.

技术参数

产品应用 ELISA;IHC;WB;IHC(P)

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