F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, PerCP-eFluor 710, eBioscience/F（ab'）2-山羊抗小鼠IgG（H + L）二抗，PerCP-eFluor 710

货号：46-4010-82,46-4010-80

规格：100 µg,25 µg

价格：1786,0

产品类型：荧光二抗

品牌：eBioscience

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：PerCP-eFluor™ 710

类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (46-4010-82) in FlowStaining of normal human peripheral blood cells with staining buffer (blue histogram) or Anti-Human CD3 Purified (a mouse IgG1 antibody, SK7,Product # 16-0036-81) (purple histogram) followed by 0.25 µg of F (ab')2 Anti-Mouse IgG PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.eLife Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain.

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