| Thermo Scientific DyLight 755 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 755 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.DyLight 755 is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 755 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor™ 750. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.DyLight 755 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry. |
| 特点 |
| •High performance—DyLight 755 replaces Alexa Fluor 755 for near-infrared staining•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency |
| 应用 |
| • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies |
| 数据 |
| Infrared Western blot detection of ERK1 using DyLight 755-conjugated secondary antibodySerial dilutions of A562 cell lysates were analyzed by Western blotting for levels of ERK1 using a mouse anti-ERK1 primary antibody and a goat anti-mouse secondary antibody conjugated to DyLight 755 (using the DyLight 755 Microscale Antibody Labeling Kit). Left lane: Infrared molecular weight marker. |
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