F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, PE, eBioscience™

货号：12-4010-82 ,12-4010-87

规格：100μg,250μg

价格：1038,2312

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：PE

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息 Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody).Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG (H+L) Secondary Antibody (12-4010-82) in FlowStaining of normal human peripheral blood cells with staining buffer (blue histogram) or Anti-Human CD3 Purified (a mouse IgG2a antibody, OKT3, Product # 14-0037-82) (purple histogram) followed by 0.125 µg of F (ab')2 Anti-Mouse IgG PE. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Nature communicationsChemo-photothermal therapy combination elicits anti-tumor immunity against advanced metastatic cancer.2.Molecular medicine reportsGenetically modified human placenta‑derived mesenchymal stem cells with FGF‑2 and PDGF‑BB enhance neovascularization in a model of hindlimb ischemia.3.NatureAllogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity.4.NatureAllogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity.5.NatureAllogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity.

技术参数

产品应用 Flow;FN;IHC

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