Goat anti-Mouse IgG F(ab')2 Secondary Antibody, FITC/山羊抗小鼠IgG F（ab'）2二抗，FITC

货号：31543

规格：2 mg

价格：2316

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：FITC

类型:	二抗	同型对照：
浓度：	1.5 mg/mL	用法：	1.5 mg/mL(ICC)；1.5 mg/mL(IF)；1:50 - 1:200(IHC)；1:50 - 1:200(IP)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Antibody Specificity: This antibody reacts with the light chains of mouse IgG and with those common to other mouse immunoglobulins based on immunoelectrophoresis. It does not react with the Fc portion of mouse IgG. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)Amax = 492 nm; Emax = 520 nmFluorophore/Protein: 9.4 µg/mg; 3.5 moles FITC per mole IgGRestoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Country of Origin: USA靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG F(ab')2 Secondary Antibody (31543) in IFImmunofluorescence analysis of Goat anti-Mouse IgG F (ab')2 Secondary Antibody, FITC conjugate was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Goat anti-Mouse IgG F (ab')2 Secondary Antibody, FITC conjugate (Product # 31543) was used at a concentration of 0.2µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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