Horse anti-Mouse IgG (H+L) Secondary Antibody, Biotin/马抗小鼠IgG（H + L）二抗，Biotin

货号：31806

规格：1.5 mg

价格：2252

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：IgG

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	1.5 mg/mL	用法：	1:500-1:5,000(ICC)；1:500-1:5,000(IF)；1:500-1:5,000(IHC)；Assay Dependent(ISH)；1:5,000-1:20,000(WB)

产品详细信息Product # 31806 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.Product # 31806 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. This antibody cross-reacts with mouse IgM (50%) hamster IgG, rat IgG (12%) rat IgM (6%) dog IgG, guinea pig IgG, swine IgG (3%) Rhesus monkey IgG (1%). It has minimal cross-reactivity with immunoglobulins from other species.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.靶标信息Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (31806) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by chemiluminescence using Horse anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Product # 31806) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106)

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