CD25 Monoclonal Antibody (PC61.5), Biotin, Functional Grade, eBioscience™

货号：36-0251-85

规格：500 μg

价格：3772

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人/小鼠

宿主：大鼠

抗体亚型：其它

克隆号：PC61.5

荧光染料：Biotin

抗体类型:	小鼠流式抗体	同型对照：	IgG1
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow)

Description:The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.05 ng/ug cytokine as determined by the LAL assay.Storage and handling: Use in a sterile environment.Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (36-0251-85)Staining of stimulated mouse splenocytes. As expected based on known expression patterns, CD25 clone PC61.5 staining is significantly increased in CD8+ T cells after stimulation. Details: Mouse splenocytes were unstimulated (blue histogram) or stimulated for 2 days with CD3 (clone 145-2C11), CD28 (clone 37.51), and Mouse IL-2 Recombinant Protein (purple histogram). After stimulation, cells were stained with CD8 (clone 53-6.7) and CD25 (clone PC61.5). Cells in the CD8+ gate were used for analysis.

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参考文献：

1.Nature immunologyProduction of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells."Published figure using CD25 monoclonal antibody (Product # 36-0251-85) in Flow Cytometry"2.EMBO molecular medicineTGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB."Published figure using CD25 monoclonal antibody (Product # 36-0251-85) in Neutralization"3.Cancer researchCombination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses.4.eLifeAffinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells."36-0251 was used in Magnetic cell separation to investigate relationship between the strength of antigenic stimulation and the response of mouse CD4+ T cells."

技术参数

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