Rat anti-Mouse IgG1 Secondary Antibody, PE-eFluor 610, eBioscience/大鼠抗小鼠IgG1二抗，PE-eFluor 610

货号：61-4015-82

规格：100 µg

价格：1822

产品类型：荧光二抗

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：PE-eFluor 610

类型:	二抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of staining with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (61-4015-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 Purified (RPA-T4, a mouse IgG1,Product # 14-0049-82) and Anti-Human CD3 APC (Product # 17-0037-42) followed by staining with 0.25 µg of Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or 0.25 µg of Anti-Mouse IgG1 PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

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