Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP/驴抗小鼠IgG (H+L)交叉吸附二抗，HRP

货号：SA1-100

规格：250 µL

价格：2278

产品类型：细胞分选

品牌：美国精骐

抗体类型:	荧光二抗	同型对照：
浓度：	40 µg/mL	用法：	1:1,000-1:20,000 (WB)

产品详细信息

Product SA1-100 is conjugated to HRP and optimized for ECL applications. This antibody reacst with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit, and sheep serum proteins.

靶标信息

Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

数据：

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (SA1-100) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by chemiluminescence using Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # SA1-100) at dilutions 1:1,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106)

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参考文献：

1.PLoS geneticsStat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation.2.The Journal of biological chemistry4-Methylumbelliferone Diminishes Catabolically Activated Articular Chondrocytes and Cartilage Explants via a Mechanism Independent of Hyaluronan Inhibition. 3.Molecular microbiology Requirement of essential Pbp2x and GpsB for septal ring closure in Streptococcus pneumoniae D39.

技术参数

产品应用 WB

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