Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, Biotin/山羊抗小鼠IgM (重链)交叉吸附二抗-生物素

货号：M31515

规格：1 mL

价格：1784

产品类型：二抗

品牌：Thermo Fisher

抗原：Mouse Mu immunonglobin

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

抗体类型:	二抗	同型对照：
浓度：		用法：	1:5,000-1:50,000 (WB);1:10,000 (IF)

产品详细信息For this goat anti-mouse IgM (Heavy chain) antibody, it is reccommended to use a 1:10,000 working dilution for optimal performance. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody (M31515) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and DU-145 (Lane 2). The blots were probed with Anti-STRO-1 Mouse Monoclonal Antibody (Product # 39-8401, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgM Heavy Chain Cross-Adsorbed Secondary Antibody, Biotin (Product # M32315) at dilutions 1:5,000 (Fig. 1), 1:20,000 (Fig. 2) and 1:50,000 (Fig. 3). A 75 kDa band corresponding to STRO-1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1.Arthritis and rheumatismAn orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus.2.The Biochemical journalConformational variation between allelic variants of cell-surface ovine prion protein. 3.The Biochemical journal Conformational variation between allelic variants of cell-surface ovine prion protein.

技术参数

产品应用 IF;WB

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