Rat anti-Mouse IgG1 Secondary Antibody, eFluor 615, eBioscience™/大鼠抗小鼠IgG1二抗，eFluor 615, eBioscience™

货号：42-4015-82,42-4015-80

规格：100 μg,25 μg

价格：3668,1467

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：M1-14D12

荧光染料：其它

抗体类型:	荧光二抗	同型对照：
浓度：	0.2 mg/mL	用法：	5 µg/mL (ICC); 5 µg/mL(IF); 5 µg/mL (IHC(P))

产品详细信息Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG1 Secondary Antibody (42-4015-82) in ICCImmunocytochemistry of fixed and permeabilized MDCK cells using 1 µg/mL Anti-alpha Tubulin Purified (left) or HeLa cells using 1 µg/mL Anti-Human Ki-67 Purified (right), followed by 5 µg/mL of Anti-Mouse IgG1 eFluor® 615.

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参考文献：

1. The Journal of biological chemistryN-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. 2.The Journal of biological chemistry N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

技术参数

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