Goat anti-Mouse IgG, IgM, IgA (H+L) Cross-Adsorbed Secondary Antibody, Biotin/山羊抗小鼠IgG，IgM，IgA（H + L）交叉吸附二抗，Biotin

货号：A-10676

规格：500 µg

价格：1795

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG/IgA/IgM (H+L)

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL(IF)；1:500-1:20,000(WB)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG, IgM, IgA (H+L) Cross-Adsorbed Secondary Antibody (A-10676) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG/IgA/IgM (H+L) Cross-Adsorbed Secondary Antibody, Biotin Conjugate (Product # A-10676) at dilutions 1:500 (Fig. 1), 1:10,000 (Fig.2) and 1:20,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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