Rat anti-Mouse IgG (Kappa light chain) Secondary Antibody, HRP/大鼠抗小鼠IgG（κ轻链）二抗，HRP

货号：MA1-34732

规格：250 µg

价格：7641

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified mouse IgG1 kappa

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：LO-MK-1

荧光染料：HRP

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.5-1 µg/mL(ELISA)；Assay Dependent(IHC (F))；1:1,000(WB)

产品详细信息Does not cross-react with other mouse immunoglobulin classes or subclasses.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (Kappa light chain) Secondary Antibody (MA1-34732) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by chemiluminescence using Rat anti-Mouse IgG (Kappa light chain) Secondary Antibody, HRP (Product # MA1-34732) at dilution 1:1,000 (Fig. 1). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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