Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP/驴抗小鼠IgG（H + L）高度交叉吸附二抗，AP

货号：A16020

规格：1 mg

价格：2724

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：驴

抗体亚型：IgG

荧光染料：AP(碱性磷酸酶)

类型:	二抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:500-1:20,000(ELISA)；1:500-1:20,000(IHC)；1:500-1:20,000(WB)

靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16020) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP conjugate (Product # A16020) at dilutions 1:500 (Fig. 1), 1:10,000 (Fig. 2), and 1:20,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product # WP20003).

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