Rabbit anti-Mouse IgG Fc Secondary Antibody/兔抗小鼠IgG Fc二抗
货号：31194
规格：2 mg
价格：2321
产品类型：二抗
品牌：Thermo Fisher
物种：小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 抗体类型: | 荧光二抗 | 同型对照： | |
| 浓度： | 2.2 mg/mL | 用法： | Assay Dependent (Flow/ICC/IF/IHC/IP/WB) |
产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31194 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31194 reacts with the heavy chains of mouse IgG but not with the light chains of most mouse immunoglobulins. This antibody does not react against mouse IgM or non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.Country of Origin: USA 靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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| 参考文献： |
| 1.The Journal of biological chemistryConservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae. |
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