Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade

货号：16-4301-81,16-4301-85

规格：50μg,500μg

价格：693,2103

产品类型：大鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：其它

类型:	大鼠同型对照	同型对照：
浓度：	1 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)；Assay-Dependent(FN)B)

产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.PloS oneA Novel Model of Asymptomatic Plasmodium Parasitemia That Recapitulates Elements of the Human Immune Response to Chronic Infection."16-4301 was used in Flow cytometry/Cell sorting to investigate aspects of the human immune response to asymptomatic persistent Plasmodium infection using a novel animal model."AuthorsFontana MF,Baccarella A,Craft JF,Boyle MJ,McIntyre TI,Wood MD,Thorn KS,Anidi C,Bayat A,Chung MR,Hamburger R,Kim CY,Pearman E,Pham J,Tang JJ,Boon L,Kamya MR,Dorsey G,Feeney ME,Kim CC2.PloS oneExperimental myocardial infarction induces altered regulatory T cell hemostasis, and adoptive transfer attenuates subsequent remodeling."16-4301 was used in Flow cytometry/Cell sorting to indicate that Tregs may serve as potential interventional targets for attenuating left ventricular remodeling."AuthorsSharir R,Semo J,Shimoni S,Ben-Mordechai T,Landa-Rouben N,Maysel-Auslender S,Shaish A,Entin-Meer M,Keren G,George J3.Journal of immunology (Baltimore, Md. : 1950)NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection."16-4301 was used in Flow cytometry/Cell sorting to investigate the cellular and molecular mechanisms of NKG2D-mediated clearance of P. aeruginosa."AuthorsWesselkamper SC,Eppert BL,Motz GT,Lau GW,Hassett DJ,Borchers MT

技术参数

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