CCL2 (MCP-1) Monoclonal Antibody (5D3-F7)

货号：14-7099-81,14-7099-85

规格：50μg,500μg

价格：1315,3514

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：5D3-F7

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-4 µg/mL(ELISA)；Assay-Dependent(Flow)；Assay-Dependent (IHC (P))；Assay-Dependent(IP)

产品详细信息Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.Applications Reported: The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections.Applications Tested: The affinity purified 5D3-F7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the biotinylated 2H5 (13-7096) antibody for detection and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The Functional Grade purified 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

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参考文献：

1.Scientific reportsMechanism and role of MCP-1 upregulation upon chikungunya virus infection in human peripheral blood mononuclear cells."14-7099 was used in Neutralization experiments to investigate the role and mechanism of MCP-1 production upon chikungunya virus infection of human peripheral blood mononuclear cells."AuthorsRuiz Silva M,van der Ende-Metselaar H,Mulder HL,Smit JM,Rodenhuis-Zybert IA2.Arteriosclerosis, thrombosis, and vascular biologyProtective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance."14-7099 was used in Flow cytometry/Cell sorting to investigate the role of B-cell functions in obesity-induced metabolic dysfunction."AuthorsHarmon DB,Srikakulapu P,Kaplan JL,Oldham SN,McSkimming C,Garmey JC,Perry HM,Kirby JL,Prohaska TA,Gonen A,Hallowell P,Schirmer B,Tsimikas S,Taylor AM,Witztum JL,McNamara CA3.Clinical biochemistryDevelopment and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform."14-7099 was used in an ELISA assay to develop a silicon photonic immunosensor for the detection of clinically-relevant concentrations of monocyte chemotactic protein 1."AuthorsValera E,Shia WW,Bailey RC4.Arteriosclerosis, thrombosis, and vascular biologyOxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes."14-7099 was used in an ELISA assay to study the role of oxidised low-density lipoprotein (oxLDL) in inducing a long lasting proinflammatory phenotype."AuthorsBekkering S,Quintin J,Joosten L5.The Journal of clinical investigationProtein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus."14-7099 was used in an ELISA assay to study how serum factor protein microarrays facilitate the detection of auto-antibody reactivity to serum factors in human samples."AuthorsPrice JV,Haddon DJ,Kemmer D,Delepine G,Mandelbaum G,Jarrell JA,Gupta R,Balboni I,Chakravarty EF,Sokolove J,Shum AK,Anderson MS,Cheng MH,Robinson WH,Browne SK,Holland SM,Baechler EC,Utz PJ

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