IL-10 Human ELISA Kit, High Sensitivity/人IL-10高灵敏ELISA试剂盒
货号:BMS215HS
规格:96 tests
价格:0
产品类型:High-Sensitivity ELISA
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | 0.05 pg/mL |
检测范围 | 0.39-25.0 pg/mL |
样本类型/体积 | 血浆 50 μL 血清 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
批间变异系数 | 7.5% |
批内变异系数 | 6.8% |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 分析缓冲浓缩液 - 生物素化检测抗体 - 放大稀释浓缩液 - 放大试剂Ⅰ - 放大试剂Ⅱ - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
Protein name | IL-10 |
Protein aliases | CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10, T-cell growth inhibitory factor |
物种(Tested) | 人 |
试剂盒类型 | High Sensitivity Sandwich ELISA Kit |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene ID | (Human) 3586 |
Gene aliases | CSIF, GVHDS, IL-10, IL10, IL10A, TGIF |
Gene symbol | IL10 |
UniProt ID | (Human) P22301 |
The Human High Sensitivity Interleukin 10 (HS IL-10) ELISA quantitates Hu HS IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-10.人白介素10(HS IL-10)高灵敏度ELISA定量了人类血清,血浆,缓冲溶液或细胞培养基中的人HS IL-10。 该检测试剂盒将专门识别天然和重组的人HS IL-10。Principle of the method 方法原理The Human HS IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人HS IL-10固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 添加了特殊的扩增试剂步骤以实现高灵敏度。 经过孵育和洗涤步骤以去除未结合物质的微孔板后,添加底物溶液,该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.白介素10是一种多效细胞因子,在调节淋巴样和髓样细胞功能中起着重要作用。在人中,IL-10由活化的CD8(+)外周血T细胞,T辅助CD4(+)T细胞克隆产生。由于IL-10具有阻断细胞因子合成的能力和巨噬细胞的几种辅助细胞功能,因此该细胞因子是巨噬细胞,T细胞和NK细胞的效应子功能的有效抑制剂。另外,IL-10参与调节B细胞,肥大细胞和胸腺细胞的增殖和分化。已经通过克隆编码细胞因子的cDNA来确定人IL-10的一级结构。相应的蛋白质具有160个氨基酸,预测分子量为18.5 kDa。根据其主要结构,IL-10是细胞因子的四个a-螺旋束家族的成员。在溶液中,人IL-10是表观分子量为39kDa的同型二聚体。尽管它包含一个N连接的糖基化位点,但它缺乏可检测的碳水化合物。因此,在大肠杆菌中表达的重组蛋白保留了所有已知的生物学活性。人IL-10基因位于1号染色体上,在基因组中以单拷贝形式存在。人IL-10与鼠类IL-10具有很强的DNA和氨基酸序列同源性,在爱泼斯坦-巴尔病毒基因组BCRF1中具有开放阅读框,它具有许多细胞因子的生物活性,因此可能在细胞因子中起一定作用。主机与病毒的交互。 IL-10的免疫抑制特性表明IL-10在抑制器官移植后移植物排斥反应方面的临床应用可能。 IL-10还可以发挥强大的抗炎活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
数据: |
IL-10 Human ELISA Kit, High SensitivityRepresentative standard curve for Human IL-10 High Sensitivity ELISA |
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