TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor 488

货号：53-7349-41,53-7349-42

规格：25 Tests,100 Tests

价格：0,2580

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：MAb11

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488
浓度：	5 µL/Test	用法：	5 µL (0.5µg)/test(Flow))

产品详细信息Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

数据

TNF alpha Antibody (53-7349-41) in FlowIntracellular staining of 5-hour PMA/Ionomycin stimulated (in the presence of brefeldin A) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) and Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human TNF alpha Alexa Fluor® 488 (right). Total cells were used for analysis.

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参考文献：

1.PloS oneApplication of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population."Published figure using TNF alpha monoclonal antibody (Product # 53-7349-42) in Flow Cytometry"2.CellHeterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient."Published figure using TNF alpha monoclonal antibody (Product # 53-7349-42) in Flow Cytometry"3.Immunologic researchThe distribution and function of human memory T cell subsets in lung cancer."Published figure using TNF alpha monoclonal antibody (Product # 53-7349-42) in Flow Cytometry"4.Human vaccines and immunotherapeuticsCell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age."Published figure using TNF alpha monoclonal antibody (Product # 53-7349-42) in Flow Cytometry"5.Science signalingTargeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma."Published figure using TNF alpha monoclonal antibody (Product # 53-7349-42) in Flow Cytometry"

技术参数

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