Rat IgG Isotype Control/大鼠IgG同型对照

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Rat IgG Isotype Control/大鼠IgG同型对照

货号:02-9602

规格:5 mg

价格:1896

产品类型:大鼠同型对照

品牌:Thermo Fisher

抗原:Gamma Immunoglobin

物种:其它

宿主:大鼠

抗体亚型:IgG

荧光染料:其它

类型:同型对照:
浓度: 2.5 mg/mL

用法:Assay-Dependent(对照试剂(Ctrl));Assay-Dependent(Flow)
靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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参考文献:
1.Oral microbiology and immunology

Chronic peroral administration of Streptococcus sobrinus to conventional laboratory rats produces cycling levels of salivary antibodies.

技术参数

产品应用 Ctrl;Flow;Misc

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