Rat IgA Isotype Control/大鼠IgA同型对照

2024-10-18

Rat IgA Isotype Control/大鼠IgA同型对照

货号:02-9488

规格:500 µg

价格:0

产品类型:大鼠同型对照

品牌:Thermo Fisher

抗原:Rat IgA

物种:其它

宿主:大鼠

抗体亚型:IgA

荧光染料:其它

类型:同型对照:
浓度: 1 mg/mL

用法:Assay-Dependent(对照试剂(Ctrl));Assay-Dependent(Flow)
产品详细信息Rat IgA was purified from ascites induced by the myeloma cells, IR 22. The protein concentration is 1 mg/mL based on E (1%) = 14 at 280 nm. 靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by nonspecific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate nonspecific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
相关产品:
▪ 流抗抗体
参考文献:
1.Parasite immunology

Variability of the intestinal immunoglobulin E response of rats to infection with Trichinella spiralis, Heligmosomoides polygyrus or Nippostrongylus brasiliensis.2.The American journal of physiology

Surfactant protein A stimulation of inflammatory cytokine and immunoglobulin production.

技术参数

产品应用 Ctrl;Flow;Misc

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