Mouse IgG Isotype Control/小鼠IgG同型对照
货号:10400C
规格:5 mL
价格:1972
产品类型:小鼠同型对照
品牌:eBioscience
抗原:Normal Mouse IgG
物种:其它
宿主:小鼠
抗体亚型:IgG
荧光染料:其它
类型: | | 同型对照: | |
浓度: | 3 mg/mL | 用法: | Assay-Dependent(对照试剂(Ctrl));Assay-Dependent(Flow) |
靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
相关产品: |
▪ 流抗抗体 |
参考文献: |
1.ASN neuro Unaltered Glutamate Transporter-1 Protein Levels in Aquaporin-4 Knockout Mice.2.Proceedings of the National Academy of Sciences of the United States of America Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. 3.Autophagy The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erbα and indirectly via Cebpb/(C/ebpβ) in zebrafish. |
技术参数 产品应用 Ctrl;Flow;IP;ChIP
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