IL-1 alpha Monoclonal Antibody (ALF-161)/IL-1 alpha单克隆抗体（ALF-161）

货号：14-7011-81,14-7011-85

规格：50 μg,500 μg

价格：1264,3539

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：ALF-161

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-4 µg/mL(ELISA)

产品详细信息Description: The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.Applications Reported: The ALF-161 antibody has been reported for use in ELISA capture.Applications Tested: The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1 alpha) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1a (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

数据

IL-1 alpha Antibody (14-7011-81) in ELISAMouse splenocytes (500,000 cells per well) were activated with LPS (1 µg/mL) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.

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参考文献：

1.Frontiers in immunologyInterleukin-1α Mediates Ozone-Induced Myeloid Differentiation Factor-88-Dependent Epithelial Tissue Injury and Inflammation.2.Nature communicationsNlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.3.European journal of immunologyNrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.4.Methods in molecular biology (Clifton, N.J.)Approaches to determine expression of inflammatory cytokines.5.Particle and fibre toxicologyThe alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles.

技术参数

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