IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), PE

货号：12-7114-80

规格：25μg

价格：1521

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：NJTEN3

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), PE
浓度：	0.2 mg/mL	用法：	2.0 µg/mL(ICC);10 µg/mL(IF);1 µg/mL(WB)

产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.Applications Tested: The PE rat IgG1 isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concntration as the experimental antibody.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.、

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参考文献：

1.The Journal of allergy and clinical immunologyConcomitant suppression of TH2 and TH17cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor γt."12-4301 was used in Flow cytometry/Cell sorting to determine the role of the TH17 cell pathway in regulating TH2 cell responses in allergic asthma."AuthorsNa H,Lim H,Choi G,Kim BK,Kim SH,Chang YS,Nurieva R,Dong C,Chang SH,Chung Y2.Oncology lettersMicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cells."12-4301 was used in Flow cytometry/Cell sorting to observe the effect on the tumour-targeting capacity of cytokine-induced killer cells, following miR-374b regulation of PD-1 expression."AuthorsHuang F,Wang B,Zeng J,Sang S,Lei J,Lu Y3.Scientific reportsRespiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4+T cells Immune Responses."12-4301 was used in Flow cytometry/Cell sorting to indicate that respiratory syncytial virus infection can increase C5a and C5aR expression in the pathogenesis of infected, asthmatic mice."AuthorsHu X,Li X,Hu C,Qin L,He R,Luo L,Tang W,Feng J4.The Journal of allergy and clinical immunologyConcomitant suppression of TH2 and TH17cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor γt."12-4301 was used in Flow cytometry/Cell sorting to determine the role of the TH17 cell pathway in regulating TH2 cell responses in allergic asthma."AuthorsNa H,Lim H,Choi G,Kim BK,Kim SH,Chang YS,Nurieva R,Dong C,Chang SH,Chung YYear2018DilutionNot Cited Oncology lettersMicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cells."12-4301 was used in Flow cytometry/Cell sorting to observe the effect on the tumour-targeting capacity of cytokine-induced killer cells, following miR-374b regulation of PD-1 expression."AuthorsHuang F,Wang B,Zeng J,Sang S,Lei J,Lu YYear2018Dilution1:50 Scientific reportsRespiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4+T cells Immune Responses."12-4301 was used in Flow cytometry/Cell sorting to indicate that respiratory syncytial virus infection can increase C5a and C5aR expression in the pathogenesis of infected, asthmatic mice."AuthorsHu X,Li X,Hu C,Qin L,He R,Luo L,Tang W,Feng JYear2017DilutionNot Cited Nature communicationsαv integrins on mesenchymal cells regulate skeletal and cardiac muscle fibrosis."12-4301 was used in Flow cytometry/Cell sorting to show that PDGFRβ+cells contribute to skeletal muscle and cardiac fibrosis via a mechanism that depends on αv integrins."AuthorsMurray IR,Gonzalez ZN,Baily J,Dobie R,Wallace RJ,Mackinnon AC,Smith JR,Greenhalgh SN,Thompson AI,Conroy KP,Griggs DW,Ruminski PG,Gray GA,Singh M,Campbell MA,Kendall TJ,Dai J,Li Y,Iredale JP,Simpson H,Huard J,Péault B,Henderson NC 5.Comparative medicineEffect of Early-life Gut Mucosal Compromise on Disease Progression in NOD Mice."12-4301 was used in Flow cytometry/Cell sorting to demonstrate that mucus reduction and subsequent increased host-bacterial contact did not affect overall disease progression in nonobese diabetic mice."AuthorsBendtsen KM,Hansen CH,Krych L,Buschard K,Farlov H,Hansen AK

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