CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), FITC, eBioscience™

货号：11-0030-81,11-0030-82 ,11-0030-85

规格：50 μg,100 μg,500 μg

价格：735,1348,2788

产品类型：流式抗体

品牌：Thermo Fisher

物种：大鼠

宿主：小鼠

抗体亚型：IgG

克隆号：eBioG4.18 (G4.18)

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Mouse IgG3 Isotype Control (B10), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test (Flow)

产品详细信息Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3 Antibody (11-0030-81) in Flow Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.25 µg of Mouse IgG3 Isotype Control FITC (Product # 11-4742-42) (left) or 0.25 µg of Anti-Rat CD3 FITC (right). Cells in the lymphocyte gate were used for analysis. CD3 Antibody (11-0030-81) PloS one 2012 -Figure 3 Inflammatory cell infiltrations and proliferation of the epithelium in the ureters of F2 mice showing hydronephrosis. Diseased ureters of BDF2 mice showed severe mononuclear cell infiltrations predominated by B220-positive cells (panel b). B220-positive cells and CD3-positive cells were mainly observed in the perivascular portions at the adventitia of ureters (panels a and b). CD16-positive cells were localized from the muscular layer to the lamina propria (panel c). Gr-1-positive cells were diffusely observed in the ureter and the lumen of gland-like structures (panel d). Metachromatic mast cells were not detected in the mucosa but several were observed in adipose tissue beside the adventitia (panel e, arrows and inset). In the pelvic-ureteral junction (panel f), proliferative cell nuclear antigen (PCNA)-positive cells were observed in the basal layer of the ureter transitional epithelium (arrows) but PCNA-positive nuclei were not detected in those of the renal pelvis (arrowheads). The black line indicates the border between the ureter and the renal pelvis (RP). UL: ureteral lumen. CD3, B220, CD16, Gr-1, PCNA: immunohistochemistry. TB: toluidine blue stain.

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参考文献：

1.BMC immunology Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy.2.Molecular medicine reports IL-17 contributed to the neuropathic pain following peripheral nerve injury by promoting astrocyte proliferation and secretion of proinflammatory cytokines.3.Spinal cord Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats.4.Molecular vision NK cell depletion delays corneal allograft rejection in baby rats.5.PloS one Molecular pathology of murine ureteritis causing obstructive uropathy with hydronephrosis.

技术参数

产品应用 Flow;IHC

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