CD5 Monoclonal Antibody (53-7.3), FITC, eBioscience™
货号：11-0051-81 ,11-0051-82 ,11-0051-85
规格：50 μg,100 μg,500 μg
价格：511,1340,2677
产品类型：流式抗体
品牌：eBioscience
抗原：CD5
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：53-7.3
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 µg/test (Flow） |
| 产品详细信息Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: The 53-7.3 antibody has been reported for use in flow cytometric analysis.<br>Applications Tested: The 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome. |
| 数据 |
CD5 Antibody (11-0051-81) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse CD5 FITC (right). Cells in the lymphocyte gate were used for analysis.CD5 Antibody (11-0051-81)eLife 2018 -Figure 1--figure supplement 1. Kindlin-3 deficiency causes thymus atrophy. ( A ) Kindlin-3 expression in protein lysates from FACS sorted CD4/CD8 double negative (DN), CD4/CD8 double positive (DP), CD4 and CD8 single positive thymocytes. GAPDH served as loading control. ( B ) Expression of kindlin-1,-2 and -3 in protein lysates of Fermt3 +/+ and Fermt3 -/- thymi. Keratinocyte and embryonic stem cell (ESC) lysates were used as positive controls for kindlin-1 and -2 expression, respectively. GAPDH was used as loading control. ( C ) Cryosections of P3 Fermt3 +/+ and Fermt3 -/- thymi were stained with anti-pan-laminin (red), anti-CD4 (green) and anti-CD8 (blue) antibodies (left panel), or with anti-pan-cytokeratin (green), anti-PECAM-1 (red) antibodies and DAPI (blue) (right panel). Cells in cyan indicate CD4/CD8 double positive cells. M, medulla; C, cortex; V, vessel; CMJ, cortico-medullary junction. Scale bar 100 um. Absolute numbers of DN, DP, CD4 + and CD8 + thymocytes present in P3 ( D ) and P6 ( E ) thymi. ( F ) Expression of TCRbeta, CD3, CD5 and CD24 on DP, CD4 + and CD8 + thymocytes from P3 Fermt3 +/+ and Fermt3 -/- thymi analysed by flow cytometry. Isotype matched controls (blue); Fermt3 +/+ cells (green); Fermt3 -/- cells (red). Bars indicate means +- standard errors. **p < 0.01; ***p < 0.001. |
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| 参考文献： |
| 1.Wellcome open research Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.2.eLife Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.3.PloS one IL-7 treatment augments and prolongs sepsis-induced expansion of IL-10-producing B lymphocytes and myeloid-derived suppressor cells.4.PloS one Naïve helper T cells with high CD5 expression have increased calcium signaling.5.Developmental cell GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia. |
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