CD8a Monoclonal Antibody (RPA-T8), FITC, eBioscience™
货号：11-0088-41 ,11-0088-42
规格：25 tests,100 tests
价格：807,1822
产品类型：流式抗体
品牌：eBioscience
抗原：CD8a
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：RPA-T8
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience |
| 浓度： | 5 µL/Test | 用法： | 5 µL (1 µg)/test (Flow） |
产品详细信息Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: The RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
| 数据 |
| CD8a Antibody (11-0088-41) in Flow Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD8a FITC (right). Cells in the lymphocyte gate were used for analysis.CD8a Antibody (11-0088-41) Gut microbes 2014 -Published figure using CD8a monoclonal antibody (Product # 11-0088-41) |
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| 参考文献： |
| 1.Stem cells international Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.2.Stem cells and development Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.3.Biomedical reportsIncreased ratio of Th17 cells to SIGIRR+CD4+ T cells in peripheral blood of patients with SLE is associated with disease activity. |
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