CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), FITC, eBioscience™

货号：11-0091-81,11-0091-82

规格：50 μg,100 μg

价格：0,1363

产品类型：流式抗体

品牌：eBioscience

抗原：CD9

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBioKMC8 (KMC8)

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	1 µg/test (Flow）

产品详细信息Description: The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naive T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcgammaRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.Applications Reported: This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

数据

CD9 Antibody (11-0091-81) in Flow Staining of BALB/c bone marrow cells with Anti-Mouse Ly-6G (Gr-1) PE (Product # 12-5931-82) and 1 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 1 µg of Anti-Mouse CD9 FITC (right). Cells in the large scatter population were used for analysis.CD9 Antibody (11-0091-81) Disease models and mechanisms 2018 -Fig. 2. Human patient-derived GSCs engraft in adult mouse SEZ. (A) Direct microinjection of human line into adult SEZ and visualization of engrafted live cells using a constitutive GFP reporter. The boxed areas are shown at higher magnification on the right. (B) Immunostaining for human-specific cytoplasmic antigen (STEM121; yellow) and KI67 (red) after 14 days. Arrowheads indicate cells immunopositive for STEM121 and KI67. (C,D), Marker analysis after 21 days of ex vivo culture: immunocytochemistry for CD133 (purple) (C) and CD9 (red) (D). Nuclear counterstaining was performed with DAPI (blue). Scale bars: 50 um in B; 20 um in C and D.

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参考文献：

1.Nature communications UBL3 modification influences protein sorting to small extracellular vesicles.2.Disease models and mechanisms Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture.3.Nature communications CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.4.Journal of immunology (Baltimore, Md. : 1950) IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

技术参数

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