CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), Functional Grade, eBioscience™/CD254 (RANK Ligand) 单克隆 (IK22/5),功能抗体, eBioscience™

货号：16-5952-85,16-5952-38,16-5952-025,16-5952-050

规格：500 µg,5 mg,25 mg,50 mg

价格：4157,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD254

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：IK22/5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The IK22/5 monoclonal antibody reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.Applications Reported: This IK22/5 antibody has been reported for use in flow cytometric analysis. The IK22/5 monoclonal antibody has also been reported to inhibit in vitro osteoclastogenesis of tartrate resistant acidic phosphatase (TRAP)+ multinucleated cells.Applications Tested: This IK22/5 antibody has been tested by flow cytometeric analysis on mouse CD254 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotickinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.仅用于科研。不用于诊断过程。

数据

CD254 (RANK Ligand) Antibody (16-5952-85)Journal of immunology (Baltimore, Md. : 1950) 2009 -Published figure using CD254 (RANK Ligand) monoclonal antibody (Product # 16-5952-85)

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参考文献：

IF1.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchSelective glucocorticoid receptor modulation maintains bone mineral density in mice."Published figure using CD254 (RANK Ligand) monoclonal antibody (Product # 16-5952-85) in Immunofluorescence"2.Journal of immunology (Baltimore, Md. : 1950)LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.

技术参数

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