CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), FITC, eBioscience™
货号：11-0111-81 ,11-0111-82 ,11-0111-85
规格：50 μg,100 μg,500 μg
价格：0,1545,2987
产品类型：流式抗体
品牌：eBioscience
抗原：CD11a
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：M17/4
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 µg/test (Flow） |
产品详细信息Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.
| 数据 |
| CD11a (LFA-1alpha) Antibody (11-0111-81) in Flow Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (blue histogram) or 0.25 µg of Anti-Mouse CD11a FITC (purple histogram). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Cell reports Repeated Antigen Exposure Extends the Durability of Influenza-Specific Lung-Resident Memory CD8+ T Cells and Heterosubtypic Immunity.2.Immunity Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.3.Toxicological sciences : an official journal of the Society of Toxicology From the Cover: Interplay Between IFN-γ and IL-6 Impacts the Inflammatory Response and Expression of Interferon-Regulated Genes in Environmental-Induced Autoimmunity.4.The Journal of clinical investigation TGF-β receptor maintains CD4 T helper cell identity during chronic viral infections.5.Shock (Augusta, Ga.) Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. |
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