Armenian Hamster IgG Isotype Control (eBio299Arm), FITC

货号：11-4888-81,11-4888-85

规格：50μg,500μg

价格：822,2580

产品类型：同型对照

品牌：eBioscience

物种：其它

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：eBio299Arm

荧光染料：FITC

类型:	Isotype Control	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Ctrl)；Assay-Dependent (Flow)；Assay-Dependent(ICC)；Assay-Dependent (IF)；Assay-Dependent(IHC)

产品详细信息Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eBio299Arm antibody has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.International journal of oncologyIntraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity."11-4888 was used in Flow cytometry/Cell sorting to evaluate the role of increased neutrophils in cancer progression."AuthorsYoshida M,Taguchi A,Kawana K,Ogishima J,Adachi K,Kawata A,Nakamura H,Sato M,Fujimoto A,Inoue T,Tomio K,Mori M,Nagamatsu T,Arimoto T,Koga K,Hiraike OW,Oda K,Kiyono T,Osuga Y,Fujii T2.International journal of nanomedicineIn vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes."11-4888 was used in Flow cytometry/Cell sorting to show that the addition of TATp to DNA-loaded liposomes increases uptake of DNA in dendritic cells."AuthorsPappalardo JS,Langellotti CA,Di Giacomo S,Olivera V,Quattrocchi V,Zamorano PI,Hartner WC,Levchenko TS,Torchilin VP3.Journal of lipid researchLipid mediator metabolic profiling demonstrates differences in eicosanoid patterns in two phenotypically distinct mast cell populations."11-4888 was used in Flow cytometry/Cell sorting to demonstrate that CTLMCs and MLMCs secrete differing oxylipin profiles at baseline and following calcium ionophore stimulation."AuthorsLundström SL,Saluja R,Adner M,Haeggström JZ,Nilsson G,Wheelock CE4.CytotechnologyInhibitory effect of hot-water extract of quince (Cydonia oblonga) on immunoglobulin E-dependent late-phase immune reactions of mast cells."11-4888 was used in Flow cytometry/Cell sorting to suggest that a crude hot-water extract of quince inhibits mast cell function during type 1 allergic reactions by suppressing inflammatory cytokine expression and lipid mediator production."AuthorsKawahara T,Iizuka T5.GlycobiologyGalectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices."11-4888 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanism by which galectin-9 inhibits lung metastasis in experimental mouse models."AuthorsNobumoto A,Nagahara K,Oomizu S,Katoh S,Nishi N,Takeshita K,Niki T,Tominaga A,Yamauchi A,Hirashima M

技术参数

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