HIF1A Human ELISA Kit/人HIF1A ELISA试剂盒
货号：EHIF1A,EHIF1A2,EHIF1A5
规格：96 Tests,2X96 Tests,5X96 Tests
价格：6021,8678,21004
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 81.92-20,000 pg/mL |
样本类型/体积 | 细胞裂解液 50 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <10% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 5X96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | HIF1A |
| Protein aliases | ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, bHLHe78, Class E basic helix-loop-helix protein 78, HIF-1, HIF-1-alpha, HIP-1, Histone-lysine N-methyltransferase SETD2, hSET2, huntingtin interacting protein 1, Huntingtin yeast partner B, Huntingtin-interacting protein 1, Huntingtin-interacting protein B, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, Hypoxia-inducible factor 1-alpha, hypoxia-inducible factor1alpha, Lysine N-methyltransferase 3A, Member of PAS protein 1, member of PAS superfamily 1, p231HBP, PAS domain-containing protein 8, Protein-lysine N-methyltransferase SETD2, SET domain-containing protein 2 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | BHLHE78, HBP231, HIF-1, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1A, HIP-1, HSPC069, HYPB, KIAA1732, KMT3A, LLS, MOP1, p231HBP, PASD8, SET2, SETD2 |
| Gene ID | (Human) 3091,(Human) 29072 |
| Gene symbol | HIF1A, SETD2 |
| UniProt ID | (Human) Q16665,(Human) Q9BYW2 |
| 数据： |
HIF1A Human ELISA Kit Representative standard curve for Human HIF-1alpha ELISA |
| The Human HIF1A ELISA quantitates Hu HIF1A in human serum, plasma, or cell culture lysates. The assay will exclusively recognize both natural and recombinant Hu HIF1A.人HIF1A ELISA定量检测人血清，血浆或细胞培养物裂解物中的人HIF1A。 该检测将专门识别天然和重组的人HIF1A。Principle of the method 方法原理The Human HIF1A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人HIF1A固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.HIF1-alpha（HIF1A）是HIF1的一个亚基，HIF1是在降低的氧气压力下培养的哺乳动物细胞中发现的转录因子。 HIF-1是由α和β亚基组成的异二聚体，均属于碱性-螺旋-环-螺旋过芳基烃受体核转运蛋白-Sim（PAS）转录因子家族。 HIF1充当对缺氧的适应性反应的转录调节因子。在缺氧条件下，HIF-1激活了40多个基因的转录，包括促红细胞生成素，葡萄糖转运蛋白，糖酵解酶，血管内皮生长因子，HILPDA和其他基因，这些蛋白质的蛋白质产物增加了氧的输送或促进了对缺氧的代谢适应。 HIF1-alpha调节缺氧介导的细胞凋亡，细胞增殖和肿瘤血管生成。缺氧诱导p53蛋白积累，与HIF1-alpha直接相互作用，并通过在缺氧条件下促进MDM2介导的泛素化和蛋白酶体降解来降低缺氧诱导的HIF1-alpha的表达。最近的研究表明，HIF1-α诱导NOX4有助于在缺氧和缺氧诱导的增殖后维持ROS水平。在人类中，它位于14号染色体的q臂上。HIF1A的C末端与p300结合。 p300 / CBP-HIF复合物参与了对缺氧反应性基因（包括VEGF）的诱导。缺氧严重影响了人类主要疾病的病理生理，包括心肌和脑缺血，癌症，肺动脉高压，先天性心脏病和慢性阻塞性肺疾病。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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