CD184 (CXCR4) Monoclonal Antibody (12G5), APC, eBioscience™
货号：17-9999-41 ,17-9999-42
规格：25 tests,100 tests
价格：1492,3189
产品类型：流式抗体
品牌：eBioscience
抗原：CD184
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：12G5
荧光染料：APC
| 抗体类型: | 流式抗体 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1 µg/test （Flow) |
| 产品详细信息Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.Applications Reported: This 12G5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of human lysed whole blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. |
| 数据 |
| CD184 (CXCR4) Antibody (17-9999-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD184 (CXCR4) APC (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1. eLife A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.2. PLoS pathogens Novel mechanisms to inhibit HIV reservoir seeding using Jak inhibitors.3. Nature communications Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells.4. Stem cells (Dayton, Ohio) ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.5. Nature communications Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. |
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