Mouse IgG2a kappa Isotype Control (eBM2a), FITC

货号：11-4724-81,11-4724-82

规格：50μg,100μg

价格：851,1387

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：FITC

类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Ctrl)；Assay-Dependent (Flow)；Assay-Dependent(ICC)；Assay-Dependent (IF)；Assay-Dependent(IHC)

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry.Applications Tested: The FITC conjugated mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.PloS oneSplenic Leukocytes Traffic to the Thyroid and Produce a Novel TSHβ Isoform during Acute Listeria monocytogenes Infection in Mice."11-4724 was used in Flow cytometry/Cell sorting to show that within three days of systemic infection of mice with Listeria monocytogenes, splenic leukocytes synthesized elevated levels of TSHβv."AuthorsMontufar-Solis D,Klein JR2.OncotargetAnti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs."11-4724 was used in Flow cytometry/Cell sorting to demonstrate a novel siRNA molecule could successfully knock down VEGF and assess its antitumor potential."AuthorsYuan D,Xia M,Meng G,Xu C,Song Y,Wei J3.iomaterialsMesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing invivo."11-4724 was used in Flow cytometry/Cell sorting to show that ADSCs are able to form tubular structures within fibrin gels, and may also contribute to faster wound healing."AuthorsMendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE4.Iranian journal of reproductive medicineComparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells."11-4724 was used in Flow cytometry/Cell sorting to investigate appropriate sources beyond embryonic stem cells to obtain germ cells and to compare the differential potentials of these cells."AuthorsHosseinzadeh Shirzeily M,Pasbakhsh P,Amidi F,Mehrannia K,Sobhani A

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-22328.htm