CD45.2 Monoclonal Antibody (104), FITC

货号：11-0454-81,11-0454-82,11-0454-85

规格：50μg,100μg,500μg

价格：822,1416,3035

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：104

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.Applications Reported: The 104 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas theyshare identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

数据

CD45.2 Antibody (11-0454-81) in FlowStaining of BALB/c splenocytes with 0.25 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (open histogram) or 0.25 µg of Anti-Mouse CD45-2 FITC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Frontiers in immunologyMicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity."11-0454 was used in Flow cytometry/Cell sorting to identify a subset of micro-RNAs that are regulated upon group-2 innate lymphoid cells (ILC2) activation, and establish a specific role for miR-155 in regulating ILC2 survival following activation."AuthorsKnolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ2.eLifeDifferential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus."Published figure using CD45.2 monoclonal antibody (Product # 11-0454-82) in Flow Cytometry"3.eLifeCCR7 defines a precursor for murine iNKT cells in thymus and periphery.AuthorsWang H,Hogquist KA4.eLifeThe H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice."Published figure using CD45.2 monoclonal antibody (Product # 11-0454-82) in Flow Cytometry"5.JCI insightParabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis."11-0454 was used in Flow cytometry/Cell sorting to examine the relative contribution of proximal tubular epithelium and circulating cells to the myofibroblast pool."AuthorsKramann R,Machado F,Wu H,Kusaba T,Hoeft K,Schneider RK,Humphreys BD

技术参数

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