CD47 Monoclonal Antibody (miap301), FITC
货号：11-0471-82
规格：100μg
价格：1492
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：miap301
荧光染料：Alexa Fluor 488
| 类型: | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), FITC |
| 浓度： | 0.5 mg/mL | 用法： | 1 μg/test(Flow) |
产品详细信息Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.No crossreactivity is seen against human CD47.Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis.Applications Tested: This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
| 数据 |
| CD47 Antibody (11-0471-82) in FlowStaining of BALB/c splenocytes with 0.5 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (blue histogram) or 0.5 µg of Anti-Mouse CD47 FITC (purple histogram). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.OncotargetBNIP3 modulates the interface between B16-F10 melanoma cells and immune cells."11-0471 was used in Flow cytometry/Cell sorting to evaluate the role played by BNIP3 in modulating the interface between B16-F10 melanoma cells and immune cells."AuthorsRomano E,Rufo N,Korf H,Mathieu C,Garg AD,Agostinis P2.Scientific reportsS. aureus blocks efferocytosis of neutrophils by macrophages through the activity of its virulence factor alpha toxin."11-0471 was used in Flow cytometry/Cell sorting to conclude that alpha toxin may contribute to tissue damage during S. aureus pneumonia by inhibiting the ability of alveolar macrophages to clear neutrophils at the site of infection."AuthorsCohen TS,Jones-Nelson O,Hotz M,Cheng L,Miller LS,Suzich J,Stover CK,Sellman BR3.Nature communicationsMtDNA mutagenesis impairs elimination of mitochondria during erythroid maturation leading to enhanced erythrocyte destruction."11-0471 was used in Flow cytometry/Cell sorting to investigate whether increased mitochondrial DNA mutagenesis underlies anaemia."AuthorsAhlqvist KJ,Leoncini S,Pecorelli A,Wortmann SB,Ahola S,Forsström S,Guerranti R,De Felice C,Smeitink J,Ciccoli L,Hämäläinen RH,Suomalainen A4.PloS oneInduction of histiocytic sarcoma in mouse skeletal muscle."Published figure using CD47 monoclonal antibody (Product # 11-0471-82) in Flow Cytometry"5.The Journal of experimental medicineThe autophagy protein Atg7 is essential for hematopoietic stem cell maintenance."11-0471 was used in Flow cytometry/Cell sorting to show that Atg7 is an essential regulator of adult hematopoietic stem cell maintenance."AuthorsMortensen M,Soilleux EJ,Djordjevic G,Tripp R,Lutteropp M,Sadighi-Akha E,Stranks AJ,Glanville J,Knight S,Jacobsen SE,Kranc KR,Simon AK |
QQ:1749072012