CD45 Monoclonal Antibody (OX1), FITC

货号：11-0461-80,11-0461-82

规格：25μg,100μg

价格：582,1474

产品类型：流式抗体

品牌：eBioscience

物种：大鼠

宿主：小鼠

抗体亚型：IgG

克隆号：OX1

荧光染料：FITC

类型:	单抗	同型对照：	CD45 Monoclonal Antibody (OX1), FITC
浓度：	0.5 mg/mL	用法：	0.25 μg/test(Flow)

产品详细信息Description: This OX1 monoclonal antibody recognizes a non-polymorphic determinant of rat CD45, which is also known as the leukocyte common antigen. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein tyrosine phosphatase exists as a variety of different isoforms due to alternative splicing.Applications Reported: This OX1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX1 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

数据

CD45 Antibody (11-0461-80) in FlowStaining of rat splenocytes with 0.125 µg of Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or 0.125 µg of Anti-Rat CD45 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Frontiers in immunologyGalectin-9 Alleviates LPS-Induced Preeclampsia-Like Impairment in Rats via Switching Decidual Macrophage Polarization to M2 Subtype."11-0461 was used in Flow cytometry/Cell sorting to establish a pre-eclampsia-like rat model by administering 1.0 μg/kg lipopolysaccharide to normal pregnant Sprague-Dawley rats via the tail vein at embryonic day 5."AuthorsLi ZH,Wang LL,Liu H,Muyayalo KP,Huang XB,Mor G,Liao AH2.Experimental and therapeutic medicineBMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cellsin vitro."Published figure using CD45 monoclonal antibody (Product # 11-0461-82) in Flow Cytometry"3.Medical science monitor : international medical journal of experimental and clinical researchTail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model."11-0461 was used in Flow cytometry/Cell sorting to study the pathway through which adipose-derived mesenchymal stem cells affect brain ischaemic injury."AuthorsChi L,Huang Y,Mao Y,Wu K,Zhang L,Nan G4.Molecular medicine reportsCD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity invitro."Published figure using CD45 monoclonal antibody (Product # 11-0461-82) in Flow Cytometry"5.Stem cell research and therapyVitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats."Published figure using CD45 monoclonal antibody (Product # 11-0461-82) in Flow Cytometry"

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-22333.htm