CD47 Monoclonal Antibody (B6H12), FITC

货号：11-0479-41,11-0479-42

规格：25 Tests,100 Tests

价格：957,2243

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号： B6H12

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (1 μg)/test(Flow)

产品详细信息Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity andregulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.This antibody has been reported to have neutralizing activity.Applications Reported: This B6H12 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This B6H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

数据

CD47 Antibody (11-0479-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD47 FITC (filled histogram). Cells in the lymphocyte gate were used for analysis. Immunohistochemistry of formalin-fixed paraffin embedded human placenta stained with 40 µg/mL Anti-Human CD47 FITC.

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参考文献：

1.OncotargetCD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis."Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry"2.Cancer medicineCD47 is an adverse prognostic factor and a therapeutic target in gastric cancer."11-0479 was used in Flow cytometry/Cell sorting to demonstrate that higher expressions of CD44 and CD47 in gastric cancer cells correlate with higher proliferation and tumorigenicity."AuthorsYoshida K,Tsujimoto H,Matsumura K,Kinoshita M,Takahata R,Matsumoto Y,Hiraki S,Ono S,Seki S,Yamamoto J,Hase K3.PloS oneAugmentation of antitumor immunity by fusions of ethanol-treated tumor cells and dendritic cells stimulated via dual TLRs through TGF-β1 blockade and IL-12p70 production."11-0479 was used in Flow cytometry/Cell sorting to illustrate the synergy between ethanol-treated whole tumor cells and dual TLRs-stimulated dendritic cells in inducing augmented CTL responses in vitro by fusion cell preparations."AuthorsKoido S,Homma S,Okamoto M,Namiki Y,Takakura K,Takahara A,Odahara S,Tsukinaga S,Yukawa T,Mitobe J,Matsudaira H,Nagatsuma K,Kajihara M,Uchiyama K,Arihiro S,Imazu H,Arakawa H,Kan S,Hayashi K,Komita H,Kamata Y,Ito M,Hara E,Ohkusa T,Gong J,Tajiri H4.PloS oneCells expressing the C/EBPbeta isoform, LIP, engulf their neighbors."Published figure using CD47 monoclonal antibody (Product # 11-0479-42) in Flow Cytometry"

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