CD48 Monoclonal Antibody (HM48-1), FITC

货号：11-0481-81,11-0481-82,11-0481-85

规格：50μg,100μg,500μg

价格：0,1545,3050

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：HM48-1

荧光染料：FITC

类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC
浓度：	0.5 mg/mL	用法：	0.25 μg/test(Flow)

产品详细信息Description: The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen; also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing a useful tool for predictionof the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells (HSC) are highly purified as CD150(+)CD244(-)CD48(-) cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244(+)CD150(-)CD48(-) and the most restricted progenitors are CD48(+)CD244(+)CD150(-). CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo.Applications Reported: This HM48-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HM48-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.

数据

CD48 Antibody (11-0481-81) in FlowStaining of BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD48 FITC (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Cell stem cellPPM1D Mutations Drive Clonal Hematopoiesis in Response to Cytotoxic Chemotherapy."11-0481 was used in Flow cytometry/Cell sorting to establish the clinical relevance of PPM1D mutations in clonal haematopoiesis and the importance of studying mutation-treatment interactions."AuthorsHsu JI,Dayaram T,Tovy A,De Braekeleer E,Jeong M,Wang F,Zhang J,Heffernan TP,Gera S,Kovacs JJ,Marszalek JR,Bristow C,Yan Y,Garcia-Manero G,Kantarjian H,Vassiliou G,Futreal PA,Donehower LA,Takahashi K,Goodell MA2.OncotargetRecipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells."Published figure using CD48 monoclonal antibody (Product # 11-0481-82) in Flow Cytometry"3.Developmental cellGATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia."11-0481 was used in Flow cytometry/Cell sorting to propose that Samd14-Enh and an ensemble of anemia-responsive enhancers are essential for erythrocyte regeneration in stress erythropoiesis."AuthorsHewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH4.Developmental cellGATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia."11-0481 was used in Flow cytometry/Cell sorting to propose that Samd14-Enh and an ensemble of anemia-responsive enhancers are essential for erythrocyte regeneration in stress erythropoiesis."AuthorsHewitt KJ,Katsumura KR,Matson DR,Devadas P,Tanimura N,Hebert AS,Coon JJ,Kim JS,Dewey CN,Keles S,Hao S,Paulson RF,Bresnick EH5.eLifeClec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton."11-0481 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts."AuthorsYue R,Shen B,Morrison SJ

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