CD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), FITC

货号：11-0493-81

规格：50μg

价格：969

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：eBioHMa5-1 (HMa5-1)

荧光染料：FITC

类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow)

产品详细信息Description: The eBioHMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.Applications Reported: This eBioHMa5-1 (HMa5-1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioHMa5-1 (HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

数据

CD49e (Integrin alpha 5) Antibody (11-0493-81) in FlowStaining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (left) or 0.5 µg of Anti-Mouse/Rat CD49e (Integrin alpha 5) FITC (right). Total viable cells were used for analysis.

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参考文献：

1.The Journal of clinical investigationαv Integrins regulate germinal center B cell responses through noncanonical autophagy."11-0493 was used in Flow cytometry/Cell sorting to identify a mechanism of regulation of germinal centre B cell TLR signalling, mediated by αv integrins and noncanonical autophagy."AuthorsRaso F,Sagadiev S,Du S,Gage E,Arkatkar T,Metzler G,Stuart LM,Orr MT,Rawlings DJ,Jackson SW,Lacy-Hulbert A,Acharya M

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