tPA Human ELISA Kit/人tPA ELISA试剂盒
货号：BMS258-2,BMS258-2TEN
规格：96 Tests,5X96 Tests
价格：6120,35426
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 6 pg/mL |
| 检测范围 | 15.6-1,000 pg/mL |
样本类型/体积 | 上清液 10 μL 血清 10 μL |
操作时间 | 1 hr 20 min |
测试时间 | 2 hr 10 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <6.5% |
| 批内变异系数（CV） | <3.6% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10X96 Tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 分析稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | tPA |
| Protein aliases | Alteplase, plasminogen/activator kringle, Reteplase, t-PA, t-plasminogen activator, tPA, Tissue-type plasminogen activator, Tissue-type plasminogen activator chain A, Tissue-type plasminogen activator chain B |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene aliases | PLAT, T-PA, TPA |
| Gene ID | (Human) 5327 |
| Gene symbol | PLAT |
| UniProt ID | (Human) P00750 |
| The Human Tissue-type Plasminogen Activator (t-PA) ELISA quantitates Hu t-PA in human serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu t-PA.人组织型纤溶酶原激活剂（t-PA）ELISA定量了人血清或细胞培养基中的人t-PA。 该检测试剂盒将专门识别天然和重组人t-PA。Principle of the method 方法原理The Human t-PA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人t-PA固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.TPA通过水解纤溶酶原中的单个Arg-Val键，将丰富但无活性的酶原纤溶酶原转化为纤溶酶。通过控制纤溶酶介导的蛋白水解，它在组织重塑和降解，细胞迁移和许多其他生理病理事件中起重要作用。特异性地裂解纤溶酶原中的Arg- -Val键形成纤溶酶，并且由A链和B链中被二硫键保持的异二聚体组成。 TPA以高亲和力与血纤蛋白结合。由于与纤溶酶原的亲和力增加，这种相互作用导致酶的催化效率提高了100到1000倍。同样，与肝素结合会增加纤溶酶原的激活。已经证明与层粘连蛋白和纤连蛋白的结合。 TPA还与甘露糖受体和低密度脂蛋白受体相关蛋白（LRP1）结合。这些蛋白质与TPA清除有关。 TPA与膜联蛋白II和细胞角蛋白8结合。迄今为止，在内皮细胞和血管平滑肌细胞（VSMC）上的相互作用尚未确定，可导致纤溶酶原激活受到100倍的刺激。此外，与VSMC的结合使PAI-1对TPA的抑制作用降低了30倍。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 数据： |
tPA Human ELISA KitRepresentative standard curve for Human T-PA ELISA |
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