CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), Super Bright 600
货号：63-0083-82
规格：100μg
价格：3133
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：Super Bright 600
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright 600 |
| 浓度： | 0.2 mg/mL | 用法： | 0.2 μg/test (Flow)； |
| 产品详细信息Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. |
| 数据 |
| CD8b Antibody (63-0083-80) in FlowC57BL/6 mouse splenocytes were stained with CD8a Monoclonal Antibody, APC (Product # 17-0081-82) and 0.125 µg of Rat IgG2b Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.125 µg of CD8b Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Neurobiology of diseasePeripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice."Published figure using CD8b monoclonal antibody (Product # 25-0083-82) in Flow Cytometry"2.PloS oneDeletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency."Published figure using CD8b monoclonal antibody (Product # 25-0083-82) in Flow Cytometry"3.Frontiers in immunologyThymic B Cell-Mediated Attack of Thymic Stroma Precedes Type 1 Diabetes Development.AuthorsPinto AI,Smith J,Kissack MR,Hogg KG,Green EA4.Nature communicationsMetastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression."Published figure using CD8b monoclonal antibody (Product # 25-0083-82) in Flow Cytometry"5.PloS oneDeletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency."Published figure using CD8b monoclonal antibody (Product # 25-0083-82) in Flow Cytometry" |
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